International O3 Congress - Oxygen Medicine:

2nd International Symposium in O3 Ozone Applications

O3 Ozone in Medicine

Ozone in Medicine - May 24th to 26th 1997

2nd International Symposium on Ozone Applications

O3 Ozone in Medicine

Ozone in Medicine - May 24th to 26th 1997

Abstracts

O3 OZONE THERAPY IN PATIENTS WITH CHRONIC GASTRITIS.

S. Androsov, L. Eremina, N. Nikolaev, B. Sarantzev, O. Maslennikov.
Nizhni Novgorod, Russia.

The aim of this work was to assess the efficiency of ozone therapy in patients with chronic gastritis. There were treated 68 patients with different forms of chronic gastritis associated with Helicobacter pylori (CGV). Every patients underwent a complex of clinico-laboratory tests, endoscopic and cytomorphologic examination.

Clinical effect was observed in 89% of cases, including improvement of endoscopic picture in 43 %, and histologically valid decrease of inflammatory activity with limitation of haemorrhages and normalization of microcirculation in gastric mucosa in 71 %.

Helicopter pylori (HP) was controlled in 29% and HP dissemination was reduced in 23 %. The tension of local inmune gastric reactions was increased in 74 % of cases.

19 patients had minimal side effects that were controlled during the first 1-3 days of treatment. According to the accompanied pathology there were developed protocols of CGV differential treatment including peroral administration of ozonated water and ozonated oil, rectal insufflations of ozone/oxygen mixture, parenteral infusions of ozonated blood and ozonated liquids, acupuncture of biologically active points with ozone/oxygen mixture. Besides there were determined indications and counterindications to ozone therapy method, the range of necessary tests and efficiency criteria.

Ozone therapy was found to be effective, non-medicament method of CGV treatment with good tolerance and easy to use.

DYNAMICS OF HISTAMINOPEXIA AND SEROTONINOPEXIA IN NEURODERMATIS PATIENTS DURING O3 OZONE THERAPY.

O. Bitkina.
Nizhny Novgorod State Medical Academy, Russia.

It is known that small doses of ozone lead to selective oxidation of the aminoacids. Sulfur contained and aromatic aminoacids are most sensitive to ozone activity. Particularly during ozone influence histidine (predecessor of histamine) level is decreasing. The increase of biogenous amines in the acute stage with heritable deficit of the plasmopexic fractions (histaminopexia and serotoninopexia).

Ozone therapy by means of minor autohemo-ozone therapy and rectal insuflactions was used for treating 60 neurodermatitis patients, 27 male and 33 female persons. Patients were from 9 to 35 years of age, 21.8 years in average. The disease lasted from 1 to 35 years (18.7 years in average).

The study of histaminopexic index (HPI) and serotoninopexic index (SPI) was made before ozone therapy course, the 2nd test was performed in 10 to 14 days of monoozone therapy and the 3rd test after the complex treatment which included traditional aids. 32 patients (53 %) demonstrated clinical remission, 27 (45 %) significant improvement and 1 patients (2 %) had small improvement.

Initial HPI level is not significant high than control group data (19.49 ± 7.04 %, the normal is 16.4 ± 1.1 %).

After 10-14 days of the monoozone therapy, HPI was increased to 30.33 ± 7.12 %. After the treatment HPI was decreased to 9.86 ± 3.69 %. Initial SPI level was 19.84 ± 1.59 % (the normal is 21.2 ±2.8 %).

In neurodermatitis, complicated by eczema, SPI level (13.49 ± 2.33 %) was significantly lower than in general group data (p‹0.05). After 10-14 days of the monoozone therapy SPI was increased to 21.12 ± 1.59 %.

After the complex treatment SPI was decreased to 18.26 ± 1.29 %. Generally SPI dynamics was like to HPI activity changes. During ozone treatment histaminopexic and serotoninopexic plasma activity increased. Decrease of that data after the treatment may be explained by elimination of connected histamine and serotonine from organism.

O3 OZONE THERAPY IN ISCHEMIC HEART DISEASE (IHD).

Y. Shaarov, N. Maslennikova, N. Dushkina, O. Maslennikov.
Regional Diagnostic Center, Nizhni Novgorod, Russia.

The aim of the work was to study the efficiency of ozone therapy in IHD patients. There were treated 85 patients, 80 having stable II-III stenocardia, 5 patients had progressive angina pectoris. The treatment course was done in 3-4 weeks.

Ozone/oxygen mixtures were administered in intraveous drops infusions and rectal insufflations. The patients condition was checked by clinical signs (number of stenocadial attacks and nitroglycerine use), tolerance to physical loads data, EKG readings, echocardioscopy.

Positive results were received in 76 patients (of 80 patients with stable stenocardia -95 %) that were on ozone therapy. Attacks of stenocardial pains were completely controlled in 46 out of 80 patients (58%) with stable stenocardia.

The amount of pain attacks was diminished in 50 % in 30 patients (37.5%). This enabled the patients to low the dose of nitrates and in a number of cases to discontinue their use. 4 patients (5 %) did not have any improvement in their condition.

Ozone therapy proved to be highly effective in patients with progressive angina pectoris and resulted in elimination of non-stable condition with significant reduction of coronary insufficiency symptoms. All the patients showed the increase to domestic and dosed physical loads. Instrumental readings had positive dynamics.

Hence, ozone therapy can be regarded as a highly efficient method of IHD treatment. Positive results are provided by ozone influence on antioxidant, coagulation and oxygen-transport systems.

O3 OZONE THERAPY IN SUPRA-SEGMENTAL VEGETATIVE DISORDERS.

S. Kotov, A. Gustov, C. Kontorschikova.
The Medical Academy of Nizhni Novgorod, Russia.

Dropping intravenous infusions of ozonated saline (OS) were used in complex treatment of 106 patients (average age 26.5) with the syndrome of permanent-paroxysmal vegetative dystonia (mean duration of 3.6 years). The most common etiological causes were neurosis (20 %), after- effects of closed cranicerebral injuries (17 %), psychoemotional tense (16 %), hereditary-constitutional factors (14 %).

Ozone therapy course included 5 procedures, administered every other day. Control group consisted of 24 patients that were on placebo treatment. 38 patients were treated with the use of double blind method. All patients were made complex dynamic examination. The results showed that dropping intravenous

OS infusions increased the efficiency of complex therapy of patients with supra-segemental vegetative disorders, decreasing resistance to the performed therapy.

OS vegetotropic action was found to depend on concentrations, OS with ozone concentration of 800 mg/L of ozone/oxygen mixtures has vegeto modifying properties. It should be adminitered in chronic post-traumatic headaches, tense headaches with signs outlow complications, dysosmia impairment, cardialgias.

OS infusions with ozone concentrations of 400 mg/L of ozone/oxygen mixtures produce sympatholytic, trophotropic effect and are recommended in chronic and episodic tense headaches, panic attacks, high level of anxiety and heliometeodependence.

Normalization of integral indexes of total cardiointerval variability and cardiac rhythm wave structure were regarded as efficiency criteria in ozone therapy of patients with supra-segmentary vegetative disorders.

Increase of superoxide dismutase activitiy in blood was regarded as biochemical efficiency criterion. Not producing significant changes in the cerebral bloodflow. OS reduces the deficiency of peripheral vasoconstriction, improves vegetative activity and thermoregulation. OS lows down the tense grade in regulatory systems function and increases defence-adjustable capacities of vegetative nervous system.

Steady normalization of vegetative tonus was observed in patients with prevalent sympathic tonus and unsteady vegetative tonus when received 5 dropping OS infusions. Patients with prevalent parasympathic tonus required repeated courses of ozone therapy to maintain the stable vegetative homeostasis.

O3 OZONE INFLUENCE ON PRO-INFLAMMATORY PROCESS IN MAXILLO-FACIAL PART OF HEAD AND NECK.

E. Durnovo, I. Kinyapina, C. Kontorschikova.
The Medical Academy of Nizhni Novgorod, Russia.

The results of ozone therapy combined with traditional treatment of 50 patients with odontogenic maxillofacial phlegmons are presented. Ozone was used in concentrations of 1300 mg/L for intravenous injections of ozonated saline and in concentration of 4500 mg/L and 2000 mg/L for local dialysis of wounds and mouthwash with ozonated distilled water. A special group consisted of patients with severe saprogenous necrotic phlegmons.

The lesions involved the neck and mediastinal organs. The surgical management of such patients often includes tracheostoma and dissection of mandibular muscles to provide a better wound drainage.

The use of ozone resulted in wound clearance from purulent exudate and necrosis on the 3-4 day. The wound was shown to have activation of mononuclear phagocytes, reinforcement of mesenchymal proliferation with parallel intensive development of epithelium covering the wound surface.

Starting from the first ozonated intravenous procedure the patients showed improvement in general condition, normalization of body temperature and laboratory blood data, activation of general antioxidant system and local immune resistance of oral cavity.

As a result secondary sutures could be done on 7-8 day and in some cases even to suture the wounds completely. It helped to avoid disfiguring scars formation.

THE USE OF O3 OZONE IN MEDICINE. CASUISTICAL ANALYSIS.

Properties which determine its industrial as well as medical use are given to ozone, allotropic variety of oxygen, characterized by triatomic molecules. Among its medical properties we can mention: antimicrobial, antiviral, antimicotic, improve the supply of oxygen to hipoxemically affected tissue areas and reactives oxygen metabolism capacities of cell, being very useful in the treatment of many pathologies.

Furthermore, its satisfactory results and its low cost, in comparison with other terapeutic agents, justify also its application.

Our study shows the casuistical analysis in 45.000 patients treated in the Ozone Therapy Department of the Provincial Hospital of Villa Clara, in a six year period, pointing out the different medical specialities and pathologies in which ozone was used as therapeutic agent alone or combined with other therapies and its efficacy.

THERAPEUTIC APPROACH WITH ALLOTROPIC OXYGEN INSUFLATION AND ACUPUNCTURE IN INFERTILITY AND IN F.I.V.E.T. A HYPOTHESIS.

G. Menaldo, M. Sodano, A. Meluzzi.
Institute of Psychosomatic Obstetric and Gynecology of Turin, Italy.

In the western world, 20 to 25 % of all couples in reproductive age appear to have reproductive problems. In our fertility and F.I.V.E.T. Centrally, we deal with infertility problems both with allopathic and natural medicine, the latter having a global approach. The combination of both seems to avoid multiple pregnancies and syndrome of ovaric hyperstimulation.

We designed therapeutic protocols which make use of acupuncture points in order to obtain an appropriate ovulation. The stimulation of 4MP, 30S, 4VC points, in the ninth day of the menstrual cycle, cause a better blood perfusion of uterus and a better endometrium trophism.

The use of allotropic oxygen in F.I.V.E.T technique seems to bring definite advantages both for reducing FSH dosage administration and in better preparing the endometrium, improving thus the change of implantation and inidation, real problem of failure in F.I.V.E.T.

Much interesting has taken place around the rectal insufflation of allotropic oxygen before embryo-transfer. It seems that from the first trials of these procedures of Global Medicine, without side effect, may bring better result. We are waiting to have a wider numbers of cases and to better study the biological and clinical researches.

O3 OZONE THERAPY ON CELULITIS DISEASE.

J.L. Cidon.
Madrid, Spain.

Celulitis is a disease due to alterations on the connective tissue. The adipocytes degenerate, growing hard, causing fiber-tissue to appear. "Orange-skin" is a celulitis characteristic, vascular weakness is another because this problem appears, in addition to other causes, due to a bad vein or limphatic flow.

The treatment must be focused on:

1st. Removing the causes of the disease.

2nd. Operate over the celulitis mechanism.

3rd. Treat the lesions caused.

Giving a mixture of oxygen/ozone in the celulitis zone causes a very important improvement in the peripherical circulation. As well, ozone is able to destroy the degenerated fat tissue (very important to fight against this problem).

USE OF OZONE THERAPY IN REITER'S SYNDROME.

Reiter's syndrome is a clinic tetrad of unknown etiology, consisting of urethritis, arthritis, mucocutaneous and conjuntivitis. It can be consecutive of an infection by chalamydia, campylobacter, salmonella or yersinia, accompanied with a systemic reaction that include fever. Occurs manily in young men and recurrences are very common.

Ozone therapy was applied in 10 out-patients of Dr. Octavio de la Concepción y de la Pedraja Hospital, in Camagüey, from 1995 to 1996, suffering from this disease. A descriptive method was used in this study. It comprised 10 male patients; the age-group that prevailed was among 20-25 years old.

The positive evolution of conjuntivitis was from the 3rd session on of ozone by rectal application.

In arthritis, 90 % had improved after the 5th session; as well as the other symptomp, showing the effectiveness as compared with the conventional treatment. There was not recurrence.

CLINIC COMPARATIVE STUDY OF O3 OZONE THERAPY AND METRONIDAZOL IN INTESTINAL AMEBIASIS.

Amebiasis is a disease, generally characterized, by dysentery with diarrhea, weakness and prostration. Also, nausea, vomithing and pain may be present. The aim of this study is to evaluate the efficiency of rectal ozone treatment in children suffering with intestinal amebiasis.

The diagnose was made by cysts or trophozoites of E. histolytica in stools. A control group using Metronidazol was used in order to compare both groups.

Ozone therapy was useful in the treatment of intestinal amebiasis with 83.8 % of efficiency and only 0.55 % of adverse effects, better than the treatment with Metronidazol who gets 71.1 % of efficiency and 17.7 % of adverse effects.

O3 OZONE THERAPY: AN USEFUL ALTERNATIVE ON VIRULENT HEPATITIS TREATMENT.

A study with 80 patients suffering from A, B, C hepatitis, since August 1996, in "Octavio de la Concepción y de la Pedraja" Hospital, Camagüey (Cuba) was performed. Patients, between 17 and 45 years old, were checked before in our laboratory, in order to verify the diagnoses of hepatitis.

Conventional treatment (repose and diet prescripted) plus ozone therapy was applied to 40 patients, the other 40 patients only received conventional treatment. Ozone was administered, daily, by rectal way (dose = 10mg), for a total of 15 sessions.

During the first week of treatment, remission of all the symptoms took place as well as the enlargement of the liver, improving their conditions and their appetite.

All patients were totally cured at the end of the treatment. Under the conventional treatment these symptoms remain for more than 10 days and the totally cure was achieved approximately 6 moths later.

Comparing these results we can say that ozone therapy is a suitable treatment against hepatitis, improving the patients health and the healing time of the disease.

SUBCUTANEOUS O3 OZONE THERAPY IN THE TREATMENT OF SIMPLEX HERPES.

Simplex herpes is a skin disease of viral etiology of difficult treatment because its recurrence, and its repeated out breaks, with lesion of erythema and vesicles, aggregated in clusters; and symptoms like burn and prurito.

After the three first days, the virus penetrates into the groups of ganglious near the lesion. It makes very difficult the recovery with the traditional treatment, because of the frequent recurrence. The treatment with subcutaneous ozone has avoided the relapses with positive results.

The research was made in a group of 84 patients with simplex herpes localized in the genitals, lips and gluteals. It has not been reported the antiviral effect of ozone in this pathology by a subcutaneous way. This make the treatment very new and safe.

OZONE THERAPY IN DERMATOSES: PYODERMA.

S.L. Krivatkin, E.V. Krivatkina.
Sormovo Interdistric Dermatovenereological Dispensary, Nizhny Novgorod, Russia.

Ozone is one of a new therapeutic agents being used in dermatology. In a recent test, 64 patients (41 men and 23 women aged 15-64) suffering from different forms of pyoderma (ecthyma-6, erysipelas-1, furunculosis-18, hidradenitis-2, impetigo-29 and sycosis barbae-8) received ozone therapy (OT). Two of its standard forms were applied: autohemotherapy minor (AHT min, intramuscular injections of 10 mL 02/03 mixture in 5 mL of autoblood) and techniques with the use of a plastic bag. As a source of 02/03 mixture the ozonizer was used. The concentration of ozone was 7-20 µg/mL. Along with OT most of the patients used traditional ointments.

Clinical recovery was achieved in 51 patients, considerable improvement in 10, improvement in 1 and no positive results in 2 patients. These data compared with the results of the previous traditional treatment (ex. systemic antibiotics) in the same 20 patients showed a better and quicker improvement in most of them.

Ozone therapy was well tolerated. It is safe to say that among the advantages of OT there are good tolerance, as well as the possibility of use in patients with candidiasis and intolerance to antibiotics, absence of pathogen resistance and relative inexpensiveness.

Howerver at this time, OT can not ensure quick results in severe pyoderma (ecthyma, etc.) when systemic antibiotics can.

Thus ozone therapy is an effective, safe and inexpensive approach to the treatment of pyoderma, it is worthy to be used.

SATURED Hb AND pO2 INVIVO AND EXVIVO IN PATIENTS TREATED WITH O3 OZONE THERAPY.

A research was made in more than 100 patients who received treatment with the method of major autohemotherapy; In these patients the Hb saturation and the partial oxygen pressure were measured before the start of the treatment and an hours after the administration of ozonized blood. There were taken also samples of the extracorporal ozonized blood. The results were compared with the samples above mentioned.

This research demonstrated that there is an important positive response, concerning gasometry, in those who received this therapy, as well as it is appreciated a great increase of tissue oxygenation. The results are shown by means of tables and graphies, including the results of extracorporal ozonization.

CORRELATION OF PLASMA INTERLEUKIN 1 LEVELS WITH DISEASE ACTIVITY IN RHEUMATOID ARTHRITIS WITH AND WITHOUT OZONE.

Z. Fahmy.
Augusta-Klinik, 55543 Bad Kreuznach, Germany.

The mean plasma level of interleukin 1 beta was significantly higher in 51 patients with rheumatoid arthritis (RA) than in 21 rheumatoid arthritis patients treated with ozone, as control group of similar age in both groups.

Plasma IL-1 beta correlated positively with Ritchie joint index, pain score, and erythrocyte sedimentation rate and correlated negatively with haemoglobin concentration. These results support the idea that IL-1 beta has a central role in the phatogenesis of RA and the ozone has influenced in the concentration level of IL-1 beta.

IMMUNOLOGICAL FINDINGS IN THE PERIPHERAL BLOOD AND IN THE SYNOVIAL FLUID AFTER INTRA-ARTICULAR O3 OZONE INJECTIONS IN RHEUMATOID ARTHRITIS.

Z. Fahmy.
Augusta-Klinik, 55543 Bad Kreuznach, Germany.

The aim of this pilot study is to find the influence that 02/03 exerts in the cytokines in peripheral blood and in synovial fluid. In this study we have two groups of classical rheumatoid arthritis. One group treated with 02/03 (major haemotherapy and intraarticular 02/03 injections), the other group without 02/03. We find there are significant changes with 02/03 (granulocytes and other cell population).

CLINICAL ASPECT OF 02/03 IN RHEUMATIC DISEASES.

Z. Fahmy.
Augusta-Klinik, 55543 Bad Kreuznach, Germany.

Among the disorders collectively named as rheumatism, progressive chronic polyarthritis constitutes a great problem, not only as regards to the frequency of its occurrence (1-2 % of the population), but also as regards its most effective therapy.

Apart from non-medication (or physical) therapy methods, long-term antirheumatics, or so called basis therapeutics, represent the only medicaments known to have effects on the pathological processes involved, going beyond a symptomatic influence, contrary to the non-steroid antirheumatics and the corticoids.

In medical ozone research over the recent year, we have arrived at results with confirm the necessity for therapeutical application of an ozone-oxygene gas mixture, not only clinically, but also biochemically.

OUR EXPERIENCES IN THE USE OF O3 OZONE THERAPY IN THE ELDERLY.

During five years (1991-1995) we have used ozone therapy in the treatment of different diseases that are present in the elderly, for example upper and lower respiratory infections, arthrosis, pneumonia, immunodeficiency.

A statistical analysis showed improvement in more than 80 % of the treated patients. No side-effects or intolerance was present among the patients treated with ozone. It is concluded that ozone therapy is an important therapeutic option to be considered in geriatric patients.

O3 OZONE THERAPY IN THE TREATMENT OF OSTEOARTHRITIS.

We have been using ozone therapy, during 5 years, in 200 patients with different types of arthritis, for example: lumbar arthritis, generalized arthrosis, hiposteoarthritis, knee arthrosis.

In our group of patients, 60 % female and 40 % of male were present (80 % with more than 40 years old). Three groups of treatment were considered: group I , using ozone therapy alone; group II, ozone therapy plus medication (antinflamatory and/or analgesics drugs) and group III, ozone therapy plus medication and physiotherapy. In general. 85 % of improvement was obtained.

O3 OZONE THERAPY IN REACTIVE ARTHRITIS.

We have used ozone therapy, like part of the treatment, of 30 patients suffering from reactive arthritis, in the last three years. No significant difference in sex distribution were present, with predominance in ages less than 45 years. Patients with reactive polyarthritis and Reiter Syndrome were included in our study.

In 55 % of patients, reactive arthritis was consequence of upper respiratory sepsis. The clinical evaluation was satisfactory, with a fast improvement and without side-effects.

O3 OZONE THERAPY IN THE TREATMENT OF BONE INFECTIONS.

It was done an investigation about the therapeutic effects of ozone therapy in the treatment of bone and articular infections, in patients suffering of these lesions, admitted to the Service of Orthopedic and Traumatology of the General Teaching Hospital "Comandante Pinares", San Cristobal, Pinar del Río.

General treatment was performed using ozone rectal application and local ozone treatment and ozonized oil (OLEOZON®). Ozone therapy directly into the septic bone was applied as a unique treatment or combined with antibioticotherapy, surgery of lesions, etc.

Good results were obtained in a great percent of patients, but an outstanding improvement was seen in patients treated with ozone as the only therapeutic method. It was well accepted by patients and no side effects were observed. It was concluded recommending its application in the treatment of chronic infections of the osteomioarticular system.

A SOLUTION FOR POST-TRAUMATIC OSTEAL EXPOSITIONS: ASSOCIATION OF GREATER OMENTUM GRAFT WITH O3 OZONE THERAPY.

Due to the frequency of injuries with postraumatic osteal exposition of the limbs, it is reported the use of greater omentum in association with ozone therapy, as a practical solution to correct these defects, with exposition of the fracture focus and in the presence or not of local infection.

It is demonstrated that with the association of these methods, healing time may be shortened, due to the early appearance of granulation tissue, even on osteal surfaces and a decrease in the inflammatory signs in a septic medium, with no signs of biological rejection.

Also, wasting material, anesthetics, drugs, and stay length may be saved, taking the graft from patients operated of puerperal ligatures, previous acceptation of donors and recipient. The graft implantation is performed with local anesthesia and there is no previous preparation of the injured patient. It is fixed with alternative sutures, its cost is very low and the patients feels highly satisfied.

It is concluded that the association of greater omentum graft with ozone therapy offers a good alternative therapeutic in the treatment of this disease.

OXYGEN OZONE O3 THERAPY AND OMOTOXICOLOGY DRUG IN GONARTHROSIS.

L. Cursio, G.Menaldo.
Unite Research of Global Medicine, I. P. O. G. Turin, Italy.

Gonarthrosis is a disease found more frequently in females ranging 2.1. Some authors maintain that this degenerative arthropathy is the most common kind of arthrosis. It is normally a bilateral condition although at its outset it present monoarticular symptoms. Traumas, congenital malformations (like: genu-valgum and genu-varum) and circulatory affections and meniscus alterations are the most frequently found causes of this diseases.

Symptoms: pain seems to be unrelated and spontaneous and it can increase when walking, but mostly when going downstairs; the joint movements are reduced and can be painful when pressure is applied to the inner side of the joint. We have looked for a combined treatment with oxygen-ozone on the acupuncture points and homotoxicology substances such as: cimicifuga, colocynthis, arnica, belladonna.

A mixture of oxygen-ozone (15 mL in all), whose concentration was 30 µg/mL, has been injected into the inter-joint space and around it (periarticulation). The patient felt a slight burning sensation which disappeared few minutes after the injection. The homotoxicology substances were thus given: arnica and cimicifuga by us, colocynthis or colocynthis belladonna by subcutaneous route around the joint.

The number of patients treated for this case study were 48, between 43 and 62 years of age. Symptoms improved rapidly (after only 3-4 treatments) and night pain was greatly reduced and so was the swelling with a return of joint functions.

The total number of treatments for patients was from a minimum of 8 to a maximum of 15, being space twice weekly first and weekly afterwards. Only 5 patients did not have a satisfactory outcome, due to their lack of cooperation.

It is concluded that although some patients did not have a total remission of the pain, nevertheless they had the great advantage of not having night pain anymore, a more confortable sleep, less swelling and improved joint movement.

OXYGEN OZONE O3 AND COLOCYNTHIS-CIMIFUGA TREATMENT IN SLIPPED DISC.

L. Cursio, G.Menaldo.
Unite Research of Global Medicine, I. P. O .G., Turin, Italy.

Low back pain presents nowadays a very serious socio-economic problem. It seems that at least once in life, 65 % of people, taken randomly from the total population, complains of serious low back, which may be further complicated into leg pain (schialgia). For this study we considered hernia of the spinal column located in L4-L5, L5-S1, because this sites represent 90 % of all back bone hernias.

Patients complain of a variety of symptoms: some having only a slight back ache in the lumbar region, others refering symptoms of serious pain, which extended to the leg, sometimes complicated by a loss of feelings sensation (paraesthesia) and hipotonic muscles.

This study comprised 73 patients. We used mixture of oxygen-ozone and colocynthis-cimicifuga injected intramuscularly around the vertebra. The mixture of 20 mL of oxygen-ozone was injected into four sites with 4 mL (total) of colocynthis-cimicifuga near vertebra l4-l5 and l5-s1, bilaterally. The pain felt after the injection, lasted only a few minutes, no side effect or complication was referred.

In 57 patients pain totally disappeared and all symptoms and muscles tone returned to normal. In 16 patients the pain improved and symptoms were reduced, but non completely suppressed.

We can say that despite 20 % of failure, 80 % of our patients was enthusiastic about the outcome, considering the small effort required of them.

THE USE OF O3 OZONE FOR THE INTENSIFICATION AND OPTIMIZATION OF ORAL HYGIENE.

L. M. Lukinikh, S. Y. Kosjuga
Russia.

Oral professional hygiene plays a great role in the prophylaxis of caries and parodontium diseases. There are a lot of data about the dependence of dental and parodontium tissues on one hand and on the quality of oral hygiene care, on the other hand.

The clinical observation have proved that the long term rational controlled oral hygiene in the background of professional hygiene gives essential prophylactic results and work out necessity to take care of teeth. The practice proves that apart from theoretical knowledge about the oral hygiene and manual habits of thorough teeth cleaning, it is important that each child and adult should have self-control criterion.

The sense of teeth smoothness may be a criterion, it can be achieved only by professional teeth cleaning. Grinding and polishing of dental and filling surfaces are being done by special brushes and abrasives pastes, skelps with fine filling and rubber during 5 minutes by horizontal, vertical and round movements, till the sense of teeth smoothness is achieved, that further determines time, technique and quality of teeth cleaning at home.

At present, ozonized distilled water (distilled water is borbotaged by ozone mixture with an ozone concentration of 2000 (g/L during 10 minutes) is widely used, for intensification and optimization of oral hygiene. It is being done by gargling with 100 mL of ozonized water per patient (3-5 passages with 20-30 mL each) during professional teeth cleaning stages.

Ozonized water increases and improved the qualitative hygienic indexes, and quantitative soft dental deposit of microbial flore composition vividly testifying to it. This investigation allows us to give recommendation about the combined usage of ozone therapy and professional oral hygiene.

They are considered to be the methods of effective influence on microbial oral landscape. They are the succesful complement and do not produce negative effects on hard dental tissues and parodontium.

EXPERIENCES WITH O3 OZONE THERAPY IN THE SUTTON DISEASE (PERIADENITIS MUCOUS NECROTICA RECURRENS). A CASE REPORT.

Sutton disease (Periadenitis Mucous Necrotic Recurrent) is a rather rare pathological condition characterized by several ulceration of the mouth, very painful, to a tendency to relapse and with a retracted healing. Many factors are involucrated in the pathogenesis of this disease, but the immgical response is one of the main aspects considered in the evolution of the pathological process.

It is presented a case of a 12-year-old age patient with diagnosis of Sutton disease with 5 years of evolution, without satisfactory results, after testing multiple therapeutic methods. The patient has been treated with ozone therapy by gas rectal insufflation (20 sessions) and local applications of ozonized sunflower oil (OLEOZON) during 2 years.

Relapses decrease during the treatment and the healing of the ulcerous injuries was more rapid and scar retractions was not observed. It is made a wide review of the topic in order to justify the application of this therapeutic method. This is the first report using ozone therapy treatment in this disease.

A COMPARATIVE STUDY OF A BACTERICIDAL ACTIVITY OF O3 OZONIZED SOLUTIONS DURING TREATMENT OF INFLAMMATORY DISEASES OF PARADONTIUM.

S. Sorokina, M. Zaslavskaja.
The Medical Academy of Nizhni Novgorod, Russia.

The problem of paradontium inflammatory diseases treatment is one of the most urgent problems in stomatology. In spite of an abundance of existing remedies and methods of treatment this problem is far from solution. It was noted periodontal pocket microflora accustomization to antibacterial preparations and allergic reactions and side effects to them.

The importance of searching new remedies becomes evident. The use of ozonized solutions for complex treatment of inflammatory diseases of paradontium is of great interest. For microbiological experiment a single irrigation of pathological periodontal pockets with ozonized solutions (water, olive oil) was performed. The pocket contents before and after irrigation were inoculated on a blood agar. Microscopic specimens were prepared simultaneously. In 18-20 hours the grown colonies were counted.

After comparison of the obtained results a substantial antibacterial activity of ozonized solutions was revealed. Number of colonies before irrigation was 48.6±5.1 and after irrigation it was 6.2 ± 0.3. the specimens show a significant decrease and qualitative changes of pathological pockets contents.

O3 OZONE THERAPY AS A PART OF A COMPLEX TREATMENT OF A PARADONTIUM DISEASE.

S. Sorokina, L. Lukinych.
The Medical Academy of Nizhni Novgorod, Russia.

Gingivitis and periodontitis are characterized by a local hypoxia of tissues and also by various microbic florae with asporogenic microorganisms and facultative anaerobes predominancy. It was demonstrated that a plaque is a part of an oral cavity microflora, but an increase of a dental deposit thickness causes changes in oral cavity ecology and leads to an anaerobiosis increase.

The ecology violation leads to gingivitis and paradontitis. In this connection an ozone therapy of the above mentioned diseases is a pathogenically sound treatment. The clinical approbation of various ozonized solutions has shown a good therapeutic effect. The solutions thanks to their oxidizing properties promote soft dental deposit, mucus, alimentary residues dissolution that is confirmed by hygienic index of Green-Vermillion.

Before treatment this index was 2.8 and after treatment 1.2. Besides, a local ozone therapy shows pronounced anti-inflammatory effects on paradontium disease, that is also confirmed by PMA index. Before treatment this index was 23 % for gingivitis, 30 % for paradontitis of a light degree, 65% for paradontitis of a middle degree.

At treatment stages using irrigation of periodontal pockets the PMA index varies substantially and decreases 2 times (18.29 accordingly). As a result of the treatment, a stomatorrhagia during gingivitis decreases or completely disappears. The volume of a gingival trevicular fluid decreases by 1.8 and 2 times accordingly.

The given therapy method is an example of an effective effect on inflammatory process in paradontium. This treatment is well endured by patients and does not cause side effects and may be used in any stomatologic institution.

APPLICATION OF O3 OZONIZED OIL IN THE TREATMENT OF ALVEOLITIS.

Septical alveolitis or septical osteitis of the alveolar cavity, after extraction is the most frequent and painful complication of exodontia. It can developed, at the third or fourth post surgical day and it is characterized by a deep pain and necrotic odor.

The etiology of the alveolitis is unknown, but the following facts increase its incidence: traumatisms, infections, vascular supply decrease in the surrounding bone and general systemic conditions. The etiology can be related to an inappropriate nutrition of the clot formed in the alveolus. Thanks to the germicide action of OLEOZON®, just as its oxygenant power, that favors the tissue regeneration, this ozonized oil was applied in the treatment of alveolitis. 100 adult patients, divided at random, in two groups of 50 patients, were studied.

To all patients, before treatments, saline solution for removing all rest of necrotic clot and/or inappropriate contents, was gently irrigated into the alveolar cavity and carefully dried with sterile swabs. Experimental group was covered, daily, with OLEOZON®. Control group was treated locally with Alvogil, also daily, in addition to oral antibiotics.

Cured was made every 72 hours and as many medical inspection as they need. Healing criterion was done, taking into account, the formation of cicatrization tissue and pain relief. Diabetes Mellitus and traumatic extractions were the factors that more favor the alveolitis appearance.

In patients treated with OLEOZON® and Alvogil, 92 % and 58 % of cured patients, respectively, were achieved, in 2 or 3 visits, with statistical significant differences between them.

In OLEOZON® group, 8 % needed 4 to 6 visits in order to complete their treatment and with Alvogil the number of patients represented 42 % Patients treated with OLEOZON® cured faster than the patients treated with Alvogil with statistical significant differences.

OLEOZON® was well tolerated by patients, without any side effect. OLEOZON® can be considered as an effective medication in the alveolitis treatment.

APPLICATION OF O3 OLEOZON® IN THE TREATMENT OF SUBPROSTHESIS STOMATITIS.

The aim of this study is to evaluate the efficacy of OLEOZON® (ozonized sunflower oil) in the treatment of Subprosthesis Stomatitis. 122 patient suffering from this disease were treated on "Briones Montoto" Dental Clinic (Pinar del Rio province) between January to April of 1996.

Patients were divided in 3 groups: group 1, 50 patients treated with OLEOZON®, without their prosthetics device; group 2, 45 patients without their prosthesis and without OLEOZON® treatment and group 3, 27 patients who maintained their prosthesis and received OLEOZON®, independently of the stomatitis degree affection. The influence of different factors: age, sex and stomatitis degree affection was statistically analyzed, through contingency tables.

The healing was total (100 %) in the first group and the time required was reduced from 43.1 (group 2) to 8.4 days, not detecting any adverse reactions. 89 % and 4 % of healing was obtained in group 2 and 3, respectively.

It is concluded that the withdrawal of the prosthesis is a very important aspect to consider and that with OLEOZON® applications the healing time is reduced significantly.

DYSCHROMIA TREATED WITH O3 OLEOZON.

A preliminary study was performed with the aim of evaluating the OLEOZON® action in the whitening of teeth, when by one reason or another they have lost their natural color.

The sample was of 30 patients, 15 patients treated with OLEOZON® and 15 patients treated with the conventional method (sodium perborate and acetone). In this study the vital teeth with dyschromia were excluded, we only treated the not vital ones or those teeth submitted to a removal of pulp.

The withening of teeth was reached from the fifth visit on using OLEOZON®. The control group had a similar behavior, so we can state that OLEOZON® substitutes sodium perborate and acetone in the treatment of dyschromia with satisfactory results.

O3 OLEOZON® IN GYNECOLOGY.

As it has been tested the germicide power, "OLEOZON®" (ozonized sunflower oil) is widely used in gynecological processes of microbial cause. It was applied daily, during 7 days, in 320 patients with micotic or bacterial Vulvovaginitis.

We demonstrated its efficiency comparing the effects of this new medicament with the conventional ones, such as "Trivagin", "Nistatine", (tables or cream) "Canesten" and Chloranphenicol ovules. We carried out vaginal exudate initially and post-treatment with successful results with the group of patients treated with "OLEOZON®". Any side-effect was report.

APPLICATION OF O3 OLEOZON® IN THE HEMORRHOIDS.

The knowledgement of the results obtained with "OLEOZON®" in the treatment of infectious-inflammatory affections and the non existence in literature of its utilization (application or use) in anal affections, constitute the central and main reason for its application in 200 patients carrying symptomatic hemorrhoids, that came to our Clinic to be assisted in "Gynecology " or "Obstetrics".

The quick disappearance of the symptoms, as well as the regression of the lesion, place "OLEOZON®" within the effective therapy for this affection so frequent in our population.

THERAPY EFFECTS OF O3 OZONIZED OIL (OLEOZON®) IN THE TREATMENT OF GIARDIASIS.

Giardia lamblia is a cosmopolite protozoan that, nowadays, creates serious problems of health because of the amount of symptoms that accompany its infection. Fifty patients from 17 to 55 years old suffering of giardiasis were studied. The diagnosis was confirmed by duodenal gavage or stool.

The patients received daily 2 capsules of OLEOZON® of 1 mL each (one in the morning and one in the night) during 10 days. Afterwards, stop treatment for 1 week and repeat the cycle the same as the first one. As healing criterion, the absence of cysts or trophozoites of Giardia lamblia, in a sample of duodenal superficial tissue under endoscopic sight, was considered.

The results demonstrated that there was 67 % of parasitologic healing and 85 % of improvement in the inflammatory process that accompanies this disease (gastritis, duodenitis).

O3 OZONIZED OIL (OLEOZON®) IN THE TREATMENT OF CHILDREN SUFFERING OF GIARDIASIS.

In the last years, an alarming increase in giardiasis, according to different reports of the Health World Organization, was achieved. The clinic symptomatology of this infection goes from cyst asymptomatic excretion to persistent and severe diarrheas, that could produce a syndrome of bad absorption. 362 children, from 8 months to 14 years, with Giardia lamblia, were studied.

The diagnosis of giardiasis was confirmed by fecal heces or duodenal gavage. OLEOZON® was dosified by drops or capsules, according to age, twice per day during 10 days, for 2 cycles of treatment with 7 days interval without medication. Approximately, 10 days after finishing the second cycle of treatment, a duodenal gavage was performed. As healing criterion, the absence of cysts or trophozoites of Giardia lamblia in fecal heces and in duodenal gavage were considered.

With the pattern of treatment proposed, 76 % of cured children and 89 % of clinical improvement (elimination of symptoms) in the remaining not cured patients were achieved.

TREATMENT OF PRIMARY PIODERMA WITH O3 OZONIZED SUNFLOWER OIL.

Taking into consideration the germicide properties of the ozonized sunflower oil (OLEOZON®), it was decided to make an investigation in 80 children between 1 and 14 year old, with the main objective of evaluating the effectiveness of the OLEOZON® in primary pyoderma, comparing this with Gentamycin cream.

The investigation was carried out in areas of health of the General Teaching Hospital "Comandante Pinares ", San Cristobal, Pinar del Rio, lasting 6 months. Patients were randomly assigned to both group of treatment; experimental (using OLEOZON®) and control (using Gentamyicin) .To all patients bacteriological studies were done, at the beginning and after 15 days of treatment.

The results demonstrated that the most frequent isolated germ was the Streptococcus b haemolytic of group A. Healing effectiveness after 15 days of treatment, was 68 % for the OLEOZON® and 28 % in those patients where Gentamycin was used. No side effect were observed.

EXPERIENCES OF NINE YEARS USING O3 OZONIZED OIL IN DERMATOLOGY.

The ozonized oils of vegetal source constitute an appropriate method for the local therapy of several diseases. The interaction of ozone with these oils produce a mixture of chemical compounds (ozonides and peroxides) with a great germicide power.

The results of topical applications of ozonized oil (OLEOZON®) in dermatological diseases of viral, fungal and bacterial origin, during 9 years of study, were presented. Among the diseases of bacterial origin (for example, pyoderma), 600 patients were treated, obtaining a healing criterion in 87 %.

According to mycotic diseases (for example, epidermophytosis, onychomycosis, etc), 1000 patients received the treatment, with 91 % of healing. In viral diseases (for example, herpes simplex), 300 patients were treated and in 72 % the relapses disappeared. Any side effect was observed.

TEN YEAR STUDY IN PATIENTS SUFFERING FROM RETINITIS PIGMENTOSA AND TREATED WITH REPEATED CYCLES OF O3 OZONE THERAPY.

Retinitis Pigmentosa is an hereditary disease of the retina which produces night blindness, peripheral visual field loss and narrowing of the central vision, which degenerates, after several years, in total blindness. This disease affects approximately 0.5 % of the world population and it is estimated that in Cuba suffer this disease, 3.1 per 10,000 habitants, with a great incidence in the central and west region of the country.

In Cuba, it has been using ozone therapy like part of the treatment of Retinitis Pigmentosa, since November 1986, in "Salvador Allende" University Hospital. A study of the clinical evolution of 20 patients that have been treated (between 5 and 10 years) with repetitive cycles of ozone therapy was performed. No other therapy was associated. The ways of ozone application was autohemotherapy or rectal insufflation.

A complete ophthalmologic examination was performed during this period. Snellen visual acuity, considering significant changes more than 2 lines and Goldmann kinetic visual field (V-4e target), considering significant changes increase in more than 10 grades and/or appearance of peripheric windows, were analyzed.

The best results were achieved when ozone treatment is repeated, at least, twice a year, along all these years, with improvement of 70 % in the visual field and 42 % in the visual acuity.

The rest of the patients maintained their visual field or visual acuity the same as the initial value, without worsen the disease. Any side effect was observed. The satisfactory results encourage to recommend its use in patients affected by Retinitis Pigmentosa.

Source: Ozone Research Center